Tet-Systems – Product Overview

Clontech

Until now, the best inducible mammalian expression systems have all required a two-vector format. Tet-One Inducible Expression Systems are the only commercially available single-vector systems that can deliver 3–4 orders of magnitude difference in expression levels between induced and uninduced states. These systems are available in lentiviral-, retroviral-, and plasmid-based formats.

• No need to create double-stable cell lines
• Obtain > 10,000-fold induction
• Viral systems include a high-titer packaging system

 

The “All-in-One” Design of Tet-One Systems

The Tet-On 3G transactivator is expressed in the forward direction from the human phosphoglycerate kinase 1 promoter (PhPGK), and the cloned gene of interest is expressed from the TRE3GS promoter (PTRE3GS) in the reverse orientation.

 

Tet-One design 

 

 

The Tet-On 3G transactivator protein is expressed constitutively from PhPGK, but is unable to bind to PTRE3GS in the absence of doxycycline (Dox; top panel). When bound by Dox (supplied in the culture medium), the transactivator undergoes a conformational change, binds to PTRE3GS, and activates transcription of a transgene cloned downstream (bottom panel).

 

Tet-On 3G Overview

Each Tet-On 3G inducible gene expression system includes two core elements:

• The inducible PTRE3G promoter, which provides the lowest basal expression of any tetracycline-inducible promoter in the absence of bound transactivator. PTRE3G retains very strong induced expression when activated by transactivator binding. 
• The Tet-On 3G transactivator, which binds to and activates expression from TRE promoters in the presence of doxycycline (Dox). Tet-On 3G is optimized for expression in human cells and for sensitivity to Dox.

 

Documents and resources

For more information visit producer's website.