Tet Systems – RNAi

Clontech

Tetracycline-Inducible miRNA

The Mir-X Inducible miRNA Red and Green Systems exploit key elements of our tightly regulated and highly responsive Tet-On Advanced Expression System to provide on-demand, high-level expression of your selected microRNA and a bright red or green fluorescent protein marker.

• Inducible microRNA expression using Tet-On Advanced
• Track your inducible miRNA expression with fluorescent protein markers
• High fold-induction and low basal expression

 

How It Works

Your microRNA sequence is embedded in the 3’ UTR of a marker protein mRNA, which is expressed from an inducible miRNA expression vector (pmRi-mCherry or pmRi-ZsGreen1). When cells expressing the Tet-On Advanced transactivator are transfected with the pmRi-mCherry or pmRi-ZsGreen1 vector, high levels of your inducible microRNA and either the mCherry or ZsGreen1 proteins are expressed in response to doxycycline (Dox). Once Dox is added to the cell culture medium, miRNA induction is very rapid and effective.

 

Red and Green Complete Systems

The complete Mir-X Inducible miRNA Systems provide the pTet-On Advanced Vector for expressing the Dox-responsive transactivator; an inducible miRNA expression vector (either pmRi-mCherry or pmRi-ZsGreen1; also available separately); two linear selectable markers (hygromycin and puromycin) for cotransfecting with the pmRi vectors in order to establish stable cell lines; and tetracycline-free Tet System Approved FBS (serum) for ensuring the full activity of Tet-based expression systems.

 

Tetracycline inducible microRNA. Tetracycline inducible microRNA. In the presence of doxycycline (Dox), Tet-On Advanced binds to the inducible promoter, PTight, and elicits high levels of transcription of a composite mRNA that encodes a fluorescent protein (mCherry or ZsGreen1), and your miRNA sequence in its 3’ UTR. Cells that fluoresce also express your microRNA

 

Documents and resources

For more information visit producer's website.